If this newsletter is not displayed correctly by your email client, please use following LINK

logo qPCR NEWS  








Gene Quantification
Q4 / 2022

is sponsored by

    Dear researcher,
Dear Gene Quantification page reader,

Since 20 years, our newsletter informs about the latest news in gene expression profiling using qPCR, dPCR, NGS, and related methods, which are compiled and summarised on www.Gene-Quantification.info

The focus of this qPCR NEWS issue is:

New Topics


Free Download

#qPCR    #MIQE 

#dPCR   #ddPCR


How to apply the MIQE guidelines - a visual, interactive and practical qPCR & dPCR guide

22 chapters authored by worldwide well-known qPCR & dPCR gurus, MIQE authors, and application specialists
Editors: Afif M. Abdel Nour & Michael W. Pfaffl
5th Edition (published on 20th February 2022)
ISBN 9783000488061

iBook, eBook & interactive PDF version for download

Free download via Apple Books (iBooks version) via https://books.apple.com/us/book/id1610947651
Free download via GQ (ePub version, 158 MB) via https://cutt.ly/8A3NpsU
Free download via GQ (PDF version, 53 MB) via https://cutt.ly/GA3N7DA both hosted by www.Gene-Quantification.info

Latest Chapters in 5th edition (Feb 2022):

Chapter 14
"Development of a high-throughput data analysis method for evaluating quantitative real-time PCR (qPCR) assays"
authored by Gregory C. Patton, Ph.D., Andrew N. Gray, Ph.D., Nathan A. Tanner, Ph.D., Janine G. Borgaro, Ph.D., Yan Xu, Ph.D., Julie F. Menin, M.S. & Nicole M. Nichols, Ph.D., New England Biolabs, Inc.

Chapter 20
"Digital Microfluidic PCR in QIAcuity Nanoplates"
written bey Kevin Matthaei, Christina Goebel, Michael Bussmann, Gerald Schock, Afif Abdel Nour, Andreas Missel; QIAGEN GmbH

Updated Chapters in 4th edition (Feb 2020):

"The influence of plastic consumables on qPCR"
written by application specialists from Eppendorf
"ddPCR – droplet digital PCR”
authored by Afif Abdel Nour
"Reproducible and Sensitive Assays using 3- and 6-colour Crystal Digital PCR™ for Detecting Point Mutations in Human Breast Cancer"
authored by scientists from Stilla Technologies
"The importance of efficiency-correction in qPCR"
authored by Jan M Ruijter & Maurice van den Hoff
"RT and qPCR Troubleshooting - How to Improve Your qPCR Data"
authored by application specialists from Analytik Jena
"Removal of between-plate variation"
written by Jan M Ruijter & Maurice van den Hoff


Call for TALK & POSTER Abstracts

10th Gene Quantification Event

20-24 March 2023



Call for TALK & POSTER Abstracts
10th Gene Quantification Event 2023

Symposium & Industrial Exhibition & Application Workshops
20 - 24 March 2023, in Freising-Weihenstephan,
School of Life Sciences, Technical University of Munich, Weihenstephan, Germany

Focus topics:   Sars-Cov-2,  Spatial-Transcriptomics,  Liquid Biopsy & CNA,  Multi-Omics Biomarkers

  • Sars-Cov-2 Quantification -- MIQE, Pitfalls and Solutions
  • Spatial-Transcriptomics
  • Multi-Omics Biomarkers
  • Liquid Biopsy & Circulating Nucleic Acids
  • Digital-PCR
  • MicroGenomics:  EV & Single-Cells Diagnostics
  • Non-coding RNAs -- microRNA, isomiRs, small-RNAs, tRFs, piRNAs, long non-coding RNAs
  • Next-Generation Sequencing
  • qPCR & dPCR & NGS -- Data Analysis, Algorithms and more ...
  • Advanced Molecular Diagnostics in Life Science
  • MIQE & Quality Management & Standardization Strategies in Molecular Diagnostics

Download #GQ2023 Event Flyer -- https://tinyurl.com/flyerGQ2023 

#GQ2023 Event Promotion Trailer -- https://youtu.be/IcOU3vosy24



Free Streaming !


Join the updated eConferences streaming webportal

Free access to over 420 recorded talks. This streaming portal is dedicated to scientists from the community of qPCR, digital PCR, Next Generation Sequencing (NGS), MicroGenomics (MG) and Molecular Diagnostics (MDx). You’ll find here all the records of the presentations held at qPCR dPCR & NGS and MG Events in the past years -- from qPCR 2010 in Vienna till GQ2019 in Freising-Weihenstephan.
We provide the presentations via movie streaming technology in high quality – high resolution and perfect sound quality in high speed – on any internet browser or mobile device.


GenEx 7.0

The most powerful tool for complex qPCR data analysis


GenEx offers advanced methods to analyze real-time qPCR data with simple mouse clicks

GenEx is the most popular software for qPCR data processing and analysis. Built in a modular fashion GenEx provides a multitude of functionalities for the qPCR community, ranging from basic data editing and management to advanced cutting-edge data analysis. GenEx 7.0 – the software compliant with MIQE and CLSI guidelines.

TATAA Biocenter, Europe´s leading provider of genomic services using quantitative real-time PCR (qPCR), and MultiD Analyses, Europe’s prime software developer for the analysis of multivariate data, release GenEx version 6 for accurate qPCR data analysi, compliant with current MIQE guideline and Clinical Laboratory Standards Institute (CLSI) guideline.

Listen to GenEx  presentations @ www.eConferences.de/MultiD/

GenEx 7.0

Compliant with MIQE and CLSI guidelines


Basic data editing and management
Arguably the most important part of qPCR experiments is to pre-process the raw data into shape for subsequent statistical analyses. The pre-processing steps need to be performed consistently in correct order and with confidence. GenEx Standard’s streamlined and user-friendly interface ensures mistake-free data handling. Intuitive and powerful presentation tools allow professional illustrations of even the most complex experimental designs.

Advanced cutting-edge data analysis
When you need more advanced analyses GenEx Enterprise is the product for you. Powerful enough to demonstrate feasibility it often proves sufficient for most users demands. Current features include parametric and non-parametric statistical tests, Hierarchical Cluster Analysis (HCA), Heatmap, Principal Component Analysis (PCA, and Artificial Neural Networks. New features are continuously added to GenEx with close attention to customers’ needs.

New features
Sample handling and samples individual biology often contribute to confounding experimental variability. By using the new nested ANOVA feature in GenEx version 5 user will be able to evaluate variance contributions from each step in the experimental procedure. With a good knowledge of the variance contributions, an appropriate distribution of experimental replicates can be selected to minimize confounding variance and maximize the power of the experimental design! For experiments with complex features, such as for example multifactorial diseases, analytical relationships and classifications may not readily be available. The support vector machine feature in the new version of GenEx is so easy to use that it will make this advanced supervised classification method easily available to novice users, while providing access to advanced parameters for experts.

View our webpage and download a FREE GenEx 7.0 trial version => GenEx.gene-quantification.info



Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages

If this newsletter is not displayed correctly by your email client, please use following LINK

The qPCR NEWS and the Gene Quantification Pages are educational sites with the only purpose of facilitating access to qPCR related information on the internet.  The qPCR NEWS and the Gene Quantification Pages are edited by Michael W. Pfaffl.   Copyright ©2004-2023    All rights reserved.   Any unauthorized use, reproduction, or transfer of this or its contents, in any medium, is strictly prohibited. Disclaimer & Copyrights are displayed on our homepage  www.gene-quantification.info
To subscribe or change your e-mail address in qPCR NEWS, and if you would like to receive future issues FREE of charge, please send an e-mail with the subject SUBSCRIBE