eSeminars
& eConference & Webinars
main topics = qPCR
& RNAi & Molecular-Biology
|
|
Illumina
introduces a new Real-Time PCR Resource Center, filled with all the
information you need to support your research.
And we mean everything, from Real-Time PCR 101 audio training modules
to data analysis guides for the experienced user, FAQs to help you
quickly resolve issues, and links to webinars where you can learn even
more.
Drop in and see all that Illumina has to offer in Real-Time PCR
=> Visit the New Real-Time PCR Resource Center
|
|
- The Genome Sequencer FLX System
- Amplicon Analysis
- Integrated Solutions Gene Knockdown
- The LightCycler® 480 System
- The Genome Sequencer 20 System
|
- Universal ProbeLibrary
- Integrated Solutions Gene Expression
Analysis
- Integrated Solutions Genotyping
- MagNAPure Compact
|
|
|
|
|
|
qPCR and MIQE Seminar Series
Sigma Aldrich Learning Center
As part of our customer education program, we have
provided two
recorded seminar series covering the topics of qPCR and MIQE. The
recorded sessions are intended to provide a high level overview of
these subject matters. We have kept the lessons concise so that you can
enjoy a self-paced learning program.
| Seminar
Title |
Presenter |
Recording
Length
(hours : minutes : seconds) |
| Primer
and Probe Design |
Ashley Heath, PhD |
0:06:32 |
| An
Introduction to qPCR Concepts |
Mudassir Mohammed, PhD |
0:09:37 |
| Selecting a
qPCR Basic Detection Chemistry |
Mudassir Mohammed, PhD |
0:12:35 |
| Choosing
a Fluorophore / Quencher Combination |
Anders Bergkvist, PhD |
0:11:30 |
| Chemistries
for More Challenging qPCR Assays |
Mudassir Mohammed, PhD |
0:15:18 |
| MIQE Concepts |
Marina Wiklander, PhD |
0:03:19 |
| Reference
Gene Validation |
Anders Bergkvist, PhD |
0:12:47 |
| Data
Analysis Guidelines |
Anders Bergkvist, PhD |
0:10:42 |
|
|
|
| Seminar
Title |
Presenter |
Recording
Length
(hours : minutes : seconds) |
| MIQE: Assay
Design Considerations |
Tania Nolan, PhD |
0:17:37 |
| MIQE: Sample
Derived Inhibitors |
Tania Nolan, PhD |
0:13:04 |
| MIQE: RNA
Quality Considerations |
Tania Nolan, PhD |
0:15:31 |
| MIQE: RNA
Quantity and RT Considerations |
Tania Nolan, PhD |
0:16:38 |
|
|
|
|
|
RNA Integrity Database (RINdb)- Bioanalyzer
RNA Profiles
Marc Valer, Microfluidics Program Manager, Genomics,
Agilent
Technologies, Santa Clara.
Synopsis:
Thousands of users today trust the bioanalyzer 2100 for
qualifying total RNA samples, looking for integrity, purity, recovery,
and consistency information for sample preparation methods, assisting
them to determine the optimal conditions for their experimental design.
Marc Valer describes the use of Agilent's new RNA Integrity Database
(RINdb) to assist in the development of experimental protocols,
particularly sample preparations, by providing a means for researchers
to contextualize RNA profiles.
|
|
A Statistical
approach to qPCR gene expression data analysis
|
|
Animation of the
Rotor-Gene 6000 on YouTube.com (Corbett
Life Science)
Further
Video on PCR or RT-PCR or real-time PCR on YouTube.com
|
|
|
|
|
|
|
|
|
Qiagen Webinars
Register for a live Webinar and hear a live talk about the cutting-edge
technologies of your choice followed by a Q&A session. The speaker
will answer as many of your questions as possible during the session.
Any remaining questions will be answered by personal e-mail after the
Webinar. Alternatively, you can listen to one of our previously
recorded Webinars.
http://www1.qiagen.com/events/webseminars/default.aspx
|
|
|
|
|
|
- miRNA purification
and detection — new tools in expression profiling and biomarker
development
- Recent progress in
RNAi screening
- A successful and
affordable RNAi solution for every lab
- Novartis scientists
share their experiences in multiplex, real-time PCR
- Transcriptome-wide
miRNA quantification by RT-PCR
- Accelerate your PCR
and qPCR
- and much
more..........................
|
|
|
|
PODCAST:
The Introduction
to Molecular and Cellular Biology lectures include
text, images, and audio. Each lecture webpage is synchronized to the
audio
component. In addition, the lectures are available as a podcast
subscription.
|
|
|
|
|
Fast
PCR and real-time PCR tutorials
(by Bio-Rad)
|
|
The
presentations listed below provide background and instruction about
specific concepts in PCR.
|
|
Free of charge web
seminars
Sigma-Aldrich
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
presentations
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
Sigma-Aldrich
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
| =>
Available Seminars: |
| Author |
Seminar
Title |
| Mudassir Mohammed, Ph.D. |
An Introduction to qPCR |
| Valerie Hawkes, Ph.D. |
Assay Design and
Optimisation |
| Neven Zoric, M.Sc. |
Approaches to Normal qPCR —
Facing the Challenge
of Normalization |
| Tania Nolan, Ph.D. |
Deriving a Troubleshooting
Protocol |
| Mikael Kubista, Prof. |
A Statistical Approach to
qPCR Gene Expression
Data Analysis |
| Marina Tshuikina, Ph.D. |
An Introduction to
Epigenetics Analysis by qPCR |
| Valerie Hawkes, Ph.D. |
Improving the
Discrimination and Sensitivity of
qPCR Assays using LNA |
| Chris Bass, Ph.D. |
Development of DNA-based
Diagnostic Assays for
Sensitive Screening of Mosquito Disease Vector Populations |
| Michael Pfaffl, Ph.D. |
Influence of RNA Integrity
on Real-Time RT-PCR
Quantification Data |
| Vladimir Benes |
microRNA Profiling |
| Rebecca Hands |
Technical Tips on LCM
Extraction for mRNA
Profiling |
| Jens Stolte |
cDNA Amplification with
GenomePlex® Complete
Whole Genome Amplification Kit |
| Tanya Novak, M.Sc. |
The SPUD Assay Has an
Important Role in the
Interpretation of Detecting Pneumocystis DNA in Clinical Specimens |
|
|
Transcriptional
Regulation of Eukaryotic Genomes
|
|
Fundamentals
of Real-Time PCR
(by
Applied
Biosystems, 47 minutes)
|
|
The Eppendorf
real-plex system
|
|
Audio
Slide Show: SmartCycler® System for qPCR
(from Cepheid)
|
|
RNA
integrity Audio slide shows
|
|
The OpenArray™
Nanotiter Plate Technology and Applications
Understanding
biological complexity arising from patterns of gene expression requires
accurate and precise measurement of RNA levels across large numbers of
genes simultaneously. The preferred method for
quantitative transcriptional analysis is real time PCR (rt-PCR) in a
96- or 384-well microtiter plate. Scaling rt-PCR to higher throughputs
is intrinsically limited by cost and logistic considerations. Alternatively
hybridization microarrays are capable of measuring transcription of
many thousands of genes simultaneously yet are limited by low
sensitivity, accuracy and sample throughput. We
demonstrate a hybrid approach by combining the superior accuracy,
precision and dynamic range of rt-PCR with the high density parallelism
of a microarray in an array of 3,072 real-time, 33 nL polymerase chain
reactions the size of a microscope slide. Real-time PCR
in this nanotiter plate format results in substantial savings in
reagents, measurement time and productivity. We demonstrate system
performance by measuring tissue-specific expression of kinase genes in
human heart and liver samples and transcriptional modulation by
small-molecule perturbation of the TNF-alpha signaling pathway in HUVEC
cells. Compared with the same assay in a 384-well
microplate, we measured equivalent rt-PCR performance with a 64-fold
reduction in assay volume, a 24-fold increase in analytical throughput
and a workflow compatible with standard microplate protocols in an
easy-to-use fomat.
|
|
Dynamic Arrays to
Measure Expression of Nucleic
Acids and Proteins
Michael Lucero of
Fluidigm speaking at AMT 2006
Click
here to launch presentation
A dynamic array is
a biochip that employs integrated
channels and valves in a matrix architecture. This matrix architecture
is a breakthrough in efficiency because performing the same set of
reactions by hand or with a robot would require orders of magnitude
more sample, reagents, and pipetting steps.Dynamic
arrays have been introduced that handle
homogeneous or heterogeneous assays, such as real-time qPCR and ELISAs,
respectively. BioMark™ dynamic arrays for qPCR accept 48 samples and 48
primer/probe sets. The components are combined into 2,304 assays (48 x
48).The chip is ideal to validate expression for 48
genes on samples from many individuals. Projected throughput of future
chips is ~10,000 reactions. BioMark™ dynamic arrays for immunoassays
accept 48 samples and 18 antibody pairs and generate 1,728 assays.
Integrated valves prevent mixing between antibody pairs.Thus,
dynamic arrays prevent signal crosstalk and
eliminate the need to optimize antibody pairs for multiplexing in one
vessel, a requirement with other formats. Instrumentation automates the
loading of chips and analysis of results. Data produced on BioMark™
dynamic arrays for both applications will be presented, demonstrating a
sensitivity of detection equivalent to conventional microwell plates
while generating orders of magnitude higher throughput.
|
|
A Sequence Oriented
Comparison of Gene Expression
Measurements Across Different Hybridization-Based Technologies
Winston Kuo of
Harvard School of Dental Medicine
speaking at AMT 2006
Click
here to launch presentation
Gene expression
microarrays have made a profound
impact in biomedical research. The diversity of platforms and
analytical methods has made comparison of data from multiple platforms
very challenging. The presentation will describe a framework for
comparisons across platforms and laboratories, where probe sequences
were utilized from each vendor to map of genes across platforms.
|
|
High-throughput
Measurement of Expression Signatures
Using Dynamic Arrays for qPCR and Immunoassays
Michael Lucero of
Fluidigm speaking at the European
Biomarkers Summit 2006
To purchase a DVD of all the presentations featured
at the European Biomarkers Summit, please go to the Select Biosciences
website.
Click
here to launch presentation
|
|
RNAi
screening – advanced tools to accelerate translational research and
drug discovery
- In
recent years, RNAi
screening using synthetic siRNA libraries has become a popular tool for
elucidating gene functional pathways and for target identification.
Find out about the essentials of RNAi screening and the latest tools
developed by QIAGEN to enable its broad application from Dr. Eric
Lader, QIAGEN’s Associate Director of RNAi research.
- RNAi screening - advanced tools to accelerate
translational research and drug discovery.
- Listen to this exciting Webinar
now.
- RNAi technology and
genome-wide expression
profiling - assessment of
specificity and pathway analysis.
- Listen to this exciting Webinar
now.
- Qiagen
Webinar web page
|
|
Identification of
New miRNA Markers for Breast Cancer with LNA Microarrays
Thomas Litman,
Exiqon, speaking at RNAi Europe 2006
Click here to launch presentation
Abnormal
expression
of microRNAs (miRNAs) in cancer implies that these small ~22-nucleotide
molecules play a role in oncogenesis, and therefore may comprise a
novel class of diagnostic and prognostic signatures. Here, we are
studying the global expression profiles of miRNAs in breast cancer and
adjacent non-malignant breast tissue in order to identify possible new
biomarkers for breast cancer
Biopsies from
primary
tumors and from the proximal tissue (1 cm and 5 cm from the border zone
of tumor) were collected from female patients (age 55-69) undergoing
surgery for invasive ductal carcinoma. Total RNA was extracted and
analyzed for global miRNA expression on a miRCURYTM LNA-based
microarray platform containing capture probes for over 400 miRNAs.
Our analysis of
miRNA
expression patterns from tumor and proximity tissue revealed numerous
differentially expressed miRNAs, including those reported to be
associated with breast cancer, such as let-7a/d/f, miR-125a/b, miR-21,
miR-32, and miR-136.
In addition, we
have
identified several miRNAs that have not previously been connected with
breast cancer. Some of these novel miRNA signatures could have
diagnostic and prognostic potential for breast cancer patients.
|
|
RNAi Based Toold in
Apptosis Research
Yu Shen, Abbott
Laboratories, speaking at RNAi Europe 2006
Click here to launch presentation
Several cases of
off-target gene silencing were identified in our siRNA library screens
(Lin X et.al. Nucleic Acids Research 33: 4527-35, 2005). However,
despite the complications of off-target gene silencing, we successfully
identified three cancer targets by screening an siRNA library against
the “druggable genome” using a cell-death assay.
In addition, in an
siRNA-based synthetic lethal screen, we found that knockdown of
survivin led to the selective killing of K-Ras mutant cells. We also
explored RNAi-based methodology for target validation in animal models.
We developed a
tightly regulated shRNA expression system (Lin X. et.al. FEBS letter,
577: 376-80, 2004) and used this system to prepare stable tumor cell
lines that conditionally expressed an shRNA for HIF-1a. These tumor
cells were implanted in mice to form tumors that served as a versatile
tool for studying the effects of inhibiting HIF-1 in vivo (Li L et.al.
Cancer Research, 65: 7249-58, 2005).
Finally, we
investigated several methods for the creation of germline knockdown
animals. By modifying the standard methods, we successfully increased
the transgenic frequency and F1 transmission rate and created
tyrosinase knockdown mice with a lighter-coat-color phenotype that can
be stably transmitted to the next generation.
|
|
OpenGenomics Peer
Science
http://www.opengenomics.com/
Welcome to
the OpenGenomics Peer Science series, where your colleagues
discuss their latest findings in Genomics research. With technical
webcasts from your peers, podcasts providing distilled takes on
research breakthroughs, and the latest in published papers, Open
Genomics is dedicated to providing fresh perspectives on pressing
research questions. Check here regularly for the latest developments in
Genomics.
Published Papers
A searchable
database of the latest
publications using Agilent's DNA
microarray platform. Search by application, date, or keyword.
Webcasts
Each webcast
contains a short scientific
talk on a specific area of
research, presented by the researcher. These webcasts are 15-20 minutes
long and are available on demand.
Podcasts
Each Podcast is
developed as an
informational brief highlighting
innovative approaches to fundamental research questions, available as
an RSS feed to your IPod®, or available for listening on your
computer.
|
|
Weekly Podcasts from
the GENcast Network
http://www.genengnews.com/gencasts.aspx
These weekly
podcasts
feature interviews with leading biotech researchers, newsmakers, and
thought leaders. Topics revolve around the critical scientific and
business issues that impact the global biotechnology industry,
beginning with new discoveries in the lab and then moving onto R &
D, biomanufacturing, and final product commercialization. Trends, novel
technologies, opinions, recent developments, how-to advice, and much
more are discussed in our podcasts in a lively, to-the-point, and
informative style. New every Thursday, you can listen right from the
GEN website or you can subscribe using the button below to have it
download each week automatically to your iPod or mp3 player!
- THE INVENTOR
OF PCR - GEN's
Editor-in-Chief John Sterling interviews the Nobel Prize winning
inventor of PCR, Dr. Kary Mullis.
(2/15/2007) sponsored by: Eppendorf
- RNAi
TECHNOLOGY
- Richard Jorgensen, Ph.D., from the University of Arizona
(3/22/2007) sponsored by: Sigma-Aldrich
- qPCR AND
LATE-PCR, AN
ADVANCED TECHNIQUE FOR DNA AMPLIFICATION - Lawrence Wangh,
Ph.D., from Brandeis University (2/22/2007) sponsored
by: Eppendorf
- PROBE-BASED,
REAL-TIME
QUANTITATIVE PCR ASSAY DESIGN AND APPLICATIONS - Gregory
L. Shipley, Ph.D., Director, Quantitative Genomics Core Laboratory, The
University of Texas Health Science Center-Houston.
(2/1/2007) sponsored by: Eppendorf
|
|
for Windows (.wmv)
for Macintosh (.mov)
for
Printing (.pdf)