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Gene
Quantification Newsletter |
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Dear researcher,
dear Gene Quantification page reader,
Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter
issue is:
-
microRNA - new links and animations
-
microRNA - updated microRNA application papers
-
webinar page - updated
-
worldwide qPCR application workshops - register now !
- qPCR Symposium USA 2008 - register now !
- 1st announcement qPCR 2009 Event => http://www.qPCR2009.net
In genetics, a miRNA (micro-RNA) is a form of single-stranded RNA which is typically 20-25 nucleotides long, and is thought to regulate the expression of other genes. miRNAs are RNA genes which are transcribed from DNA, but are not translated into protein. The DNA sequence that codes for an miRNA gene is longer than the miRNA. This DNA sequence includes the miRNA sequence and an approximate reverse complement. When this DNA sequence is transcribed into a single-stranded RNA molecule, the miRNA sequence and its reverse-complement base pair to form a double stranded RNA hairpin loop; this forms a primary miRNA structure (pri-miRNA).The function of miRNAs appears to be in gene regulation. For that purpose, a miRNA is complementary to a part of one or more messenger RNAs (mRNAs). Animal miRNAs are usually complementary to a site in the 3' UTR whereas plant miRNAs are usually complementary to coding regions of mRNAs. The annealing of the miRNA to the mRNA then inhibits protein translation, but sometimes facilitates cleavage of the mRNA. This is thought to be the primary mode of action of plant miRNAs. In such cases, the formation of the double-stranded RNA through the binding of the miRNA triggers the degradation of the mRNA transcript through a process similar to RNA interference (RNAi), though in other cases it is believed that the miRNA complex blocks the protein translation machinery or otherwise prevents protein translation without causing the mRNA to be degraded. miRNAs may also target methylation of genomic sites which correspond to targeted mRNAs. miRNAs function in association with a complement of proteins collectively termed the miRNP.
http://microRNA.gene-quantification.info
- microRNA Definition
- non-coding RNAs & non coding RNA DataBase
- micro RNA talks & microRNA posters
- Nature REVIEWS & Nature Genetics - The microRevolution
- miRNA Research Education Research Center
- Human microRNA targets
- Cancer genomics: Small RNAs with big impacts
- miRU: an automated plant miRNA target prediction server
- miRBase - the home of microRNA data
- The microRNA Registry
- A uniform system for microRNA annotation
- TRC - the RNAi Consortium shRNA Library
- M. Zuker Research: Algorithms & Thermodynamics for Nucleic Acid Folding, Hybridization & Melting Profile Prediction
- TUTORIAL: Check out an illustrated tutorial on miRNA (by Ambion)
- Publications from the Victor Ambros lab
- => Quantitative real-time RT-PCR applications for microRNA quantification
updated
publications
Updated microRNA real-time RT-PCR publications:
- Strategies to determine the biological function of microRNAs.
- Genomics of microRNA.
- Tracing microRNA patterns in mice.
- Target mimics modulate miRNAs.
- Target mimicry provides a new mechanism for regulation of microRNA activity.
- Lessons from Nature: microRNA-based shRNA libraries.
- Identification of Differentially Expressed MicroRNAs by Microarray.
- Rational Probe Optimization and Enhanced Detection Strategy for MicroRNAs Using Microarrays.
- Potential mRNA Degradation Targets of hsa-miR-200c, Identified Using Informatics and qRT-PCR.
- Multiplexing RT-PCR for the detection of multiple miRNA species in small samples.
- An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues.
- Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs.
- Glimpses of a Tiny RNA World.
- Functions of microRNAs and related small RNAs in plants.
- Evidence that microRNAs are associated with translating messenger RNAs in human cells.
- How microRNAs control cell division, differentiation and death.
- microRNA target predictions in animals.
- Computational identification of microRNA targets.
- Incorporating structure to predict microRNA targets.
- 220-plex microRNA expression profile of a single cell.
- Target labelling for the detection and profiling of microRNAs expressed in CNS tissue using microarrays.
Update of the qPCR Webinar page
A lot of
interesting WEBINARs, SLIDE SHOWs, and PODCASTs from various speakers,
biotec companies, qPCR Events, and international journals (Nature and
Science) are FREE for download. Have a look and you will definitely
something interesting for your scientific work !
=> http://webinar.gene-quantification.info/
World-wide
academic and commercial qPCR Events
Events.gene-quantification.info
Symposia, Meetings,
Conferences, Workshops, Seminars,
Online-Seminars, qPCR Education Program, ...etc.
Please submit your qPCR event here =>
events@gene-quantification.info
qPCR Symposium USA 2008
10. - 13. November 2008
Clarion Hotel San Francisco Airport, Millbrae, CA , USA
Symposium
Focus:
Markers, Stem
Cells, Single
Cell, siRNA, miRNA, Diagnostics, Immuno-qPCR, Expression
Profiling,
Poster Presentation, Workshops in qPCR hosted by the TATAA
Biocenter http://www.tataa.com/
qPCR 2009
EVENT
9 -
13 March 2009
more news here
=>
TATAA Biocenter Germany - qPCR Application workshops
At the TATAA
Biocenter Germany we offer qPCR application
workshops, a 3-day qPCR Core Module and a 2-day qPCR Biostatistics Module. All courses are held regularly in Göteborg, Sweden, in English and in Freising-Weihenstephan, Germany,
in German and English, and in Prague,
Czech Republic in English and Czech.
Depending on the occasion the workshop language and the different
prices may apply. Further customized workshops and
specialized trainings will be held as well across Europe and world-wide.
TATAA Biocenter Germany workshops are held in
cooperation with bioEPS GmbH,
located at the campus of the Technical University of Munich, in
Freising-Weihenstephan, very close to the Munich Airport (MUC). For more information and to register for the qPCR
application workshops, please see our web page => http://tataa.gene-quantification.info/
Course Occasions 2008:
3-day qPCR Core Module (Mon. - Wed.) and 2-day BioStatistics Module (Thu. - Fri.)
13-17 Oct Prague
RNA Isolation + qPCR Core Module + HRM - 13-17 Oct Freising Germany qPCR Core Module + Biostatistics (Kurs wird in DEUTSCH gehalten, German language)
- 27-31 Oct Göteborg qPCR Core Module + HRM + Biostatistics
- 17-21 Nov Prague qPCR Core Module + Practical Biostatistics
- 24-28 Nov Freising Germany qPCR Core Module + Biostatistics (English language)
- 1-5 Dec Göteborg qPCR Core Module + Biostatistics
- 15-19 Dec Prague RNA Isolation + Expression Profiling and Data Analysis
Download list of TATAA courses in autumn and fall 2008
Please register here => http://www.tataa.com/Courses/Courses.html
Best regards,
Michael W. Pfaffl
responsible Editor of the Gene
Quantification Pages
http://www.gene-quantification.info
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