qPCR Workshops:
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Our highly appreciated courses are suitable for
researchers,
technicians, post-graduate students or anyone that wants to learn more
about real-time PCR and real-time PCR related technologies. Most of our
courses have both theoretical and practical parts where the
participants
get to do experiments themselves under experienced supervision.
Different course modules are available at different occasion and you
may take a full week course or just a single course module
=> qPCR
course event calendar
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Title:
Principles,
parameters and applications of PCRDate:
28 February 2012 (code
2545ALS2012)
Course leader:
Dr. M.C.M. Verschuren, Avans University of Applied Sciences, NL
Location: Avans
University of Applied Sciences
Costs
€ 450,--
Title:
Real-Time PCR in molecular diagnostics
Data:
29 February till 2 March 2012 (3
days, code 2548ALS2012)
Course
leader: Dr. M.C.M. Verschuren, Avans University of Applied
Sciences, NL; Dr. O. Bakker, qPCR, NL
Location: Avans
University of Applied Sciences
Costs: €
1080,--
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qPCR Experience Real-Time PCR workshop in
Plant Pathology - Diagnostics and Research
29. Nov 2011 - 2. Dec 2011
Workshop description
This 4-day workshop will cover real-time PCR in diagnostics and
research in the field of plant pathology and is organised by
Biosistemika Ltd. and National Institute of Biology.
Instructors with years of experience in development and use of
real-time PCR will provide theoretical background and comprehensive
hands-on training in:
• setting-up experiments
• sample processing
• data analysis
• quality control
• validation of methods under ISO standards.
Hands on
Plant pathology specialists from National Institute of Biology will
also help to lead extensive practical laboratory part that will include
plant sample preparation, nucleic acid extraction and real-time PCR
reaction set-up as well as results discussion and interpretation also
through examples.
Instructors and lecturers
qPCR instructors will include plant pathology specialists from the
Department of Biotechnology and Systems Biology at National Institute
of Biology.
Guest lecturer: Dr. Neil Boonham, the team leader at Fera (The Food and
Environment Research Agency, York, United Kingdom) and world known
specialist for development of new molecular tools (qPCR, microarrays,
LAMP, new generation sequencing) in microbiology. He will share his
knowledge and experience in his lecture about new approaches to plant
pathogens diagnostics.
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Accuracy in Molecular Measurement Training
Course - Gene Expression Analysis
- Event Type - Training Course
- Location - LGC, Teddington
- Start Date - 2 November 2011
- Duration - 2 days
- Cost - £750 + VAT
Learn how to perform reliable gene expression
analysis and about the strategies that can be adopted for ensuring that
you are generating robust and accurate results.
On 2-3 November 2011 LGC, in collaboration with
Sigma-Aldrich, will be providing training in gene expression analyses.
This will focus on the latest approaches to molecular analyses of RNA
expression, including next generation sequencing, and discuss the
essential considerations needed to ensure confidence in RNA
quantification.
Presenters:
- Professor Stephen Bustin (Barts and the London
School of Medicine and Dentistry, Queen Mary University of London)
- Dr Vladimir Benes (EMBL Heidelberg)
- Dr Tania Nolan (Sigma-Aldrich)
- Dr Jim Huggett (LGC)
For more information
and registration details, see the training course flyer
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qPCR Symposia, Meetings, and
Conferences:
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TATAA Biocenter and PhileKorea Technology
proudly presents and welcomes you to the qPCR and Next Generation
Sequencing symposium of 2012
taking place in Daejeon, South Korea, November
13th-16th, 2012
The
symposium
will consist of days with scientific sessions and
industrial exhibition and also symposium workshops.
Besides interesting talks about the new developments in real-time
qPCR
and Next Generation Sequencing (NGS) research, special focus in this
symposium is:
Symposium Topics:
* Molecular diagnostics
* Standardization and quality control
* Preanalytics: sample preparation, handling and
storage of clinical specimens
* Molecular Biomarkers
* High throughput expression profiling
* Epigenetics, mutation analysis and copy number
variation
* Single-cell and subcellular expression profiling
* Non coding RNAs: RNAi and miRNA
* Detection and characterization of tumor cells
* qPCR and NGS in Forensics and AgriBio
* Experimental design and data mining
* Clinical applications of qPCR and NGS
* Digital PCR
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Molecular
Diagnostics: from
single-cells to Next
Generation
Sequencing
Leading
academic researchers and
industrial contributors in the field will participate in the
symposium, which will be an arena for fruitful discussions between
researchers of different backgrounds. The Symposium Talks, Poster
Sessions, Industrial
Exhibition and associated qPCR Application
Workshops offer an
overview of
the present knowledge and future developments in qPCR and gene
expression measurement technology and
its wide applications.
The
symposium
will
focus on 60-70 lectures and more than 100 posters will be presented
by
internationally
recognised experts in their field. The emphasis will be
on unbiased, didactic
information exchange. Internationally
reknown speakers will be participating in a lively and exciting
programme enabling the valuable exchange of information in the qPCR
field. One
third
of
the talks
will
be
presented by selected invited speakers,
one
third will be selected from the submitted
abstracts and one
third will be presented by qPCR related company
R&D
representatives. All
scientific contributions will be published
in the qPCR
2011
Symposium
Proceedings. Download symposium flyer
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All
symposium participants have FREE access
for a
limted time to the recorded
talks and to the updated online proceedings including all participants
contact data.
The talks by the
plenary
lecturer, selected invited
academic and industrial speakers will be published in METHODS special
qPCR Vol 50 issue 4 (April 2010) with
the title “The
ongoing
evolution of qPCR”.
At the meeting all
participants will get a free hard cover of the issue => please
view the TOC
and HTML
or PDF versions
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Single-Cell
Gene Expression Analysis
EMBO Practical Course - EMBL Heidelberg, Germany
Monday 19 March - Friday 23 March 2012
Why You Should Apply
Summary
The molecular analysis of limited biomass from a single cell type can
broadly be defined as Microgenomics. This technique is often used to
increase the knowledge in areas such as cancer development, plant
physiology, neurodegenerative disorders or developmental biology, where
the analysis of transcripts in minute samples are necessary. For the
success of the technique, homogenous samples of the cell types to be
analysed are of primary importance, making a very precise sampling
crucial. Depending on the nature of the sample and the goal of the
experiment, there are different ways of sampling the probes. This
course will present flow cytometry and laser capture microdissection as
methods to obtain single cells for the subsequent gene expression
analysis. The need for quantitative single-cell mRNA analysis is given
by the inability of conventional RNA methods, like northern blotting,
RNAse protection assay or classical block RT-PCR, to differentiate mRNA
abundance of such limited samples. Another crucial step is the data
analysis, which also constitutes a strong component of the course.
Topics:
- Laser Capture Microdissection
- Flow Cytometry
- Single-Cell qRT-PCR
- Data Analysis
Speakers & Trainers:
- Claudia Bevilacqua, INRA, France
- Shirley Chu, Life Technologies, USA
- Alison Devonshire, LGC Limited, United
Kingdom
- Jacqueline Dreyer, EMBL Heidelberg,
Germany
- Mikael Kubista, Institute of
Biotechnology, Czech Republic
- Birgit Liss, University of Ulm, Germany
- Christoph Merten, EMBL Heidelberg,
Germany
- Michael W. Pfaffl, Technical University
Munich, Germany
- Andrew Riddell, EMBL Heidelberg, Germany
- Vendula Rusňáková,
Institute of Biotechnology, Czech Republic
- Anders Ståhlberg, University of
Gothenburg, Sweden
- Raimo Tanzi, Life Technologies, Italy
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Advanced qPCR Techniques for Publication Success:
Following MIQE
Recommendation
EMBL Heidelberg, Germany Monday 2 July - Friday 6 July 2012
Why You Should Apply
Since the early descriptions of the use of
quantitative Real Time PCR, the technique has been adopted in almost
every aspect of life science research and is increasingly used for
clinical analysis. Over time protocols and strategies have been tried
and tested, amended and developed such that there are currently several
different approaches. Protocol variations are evident at each step of
the RT-qPCR process, from sample acquisition to data analysis (e.g.
sample QC, experimental design, assay design and validation,
normalisation, biostatistical interpretation, reporting, etc). It is
now apparent that these adaptations may result in differences in the
final biological conclusion of the study.
Each step of the RT-qPCR will be discussed and
the variability in resulting data that is due to different protocols
will be illustrated.
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qPCR
Seminars and Education Programs:
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Free
of charge web
seminars
Sigma-Aldrich
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
presentations
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
Sigma-Aldrich
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
Expert
speakers
will cover areas such as:
- QPCR assay design and
optimisation
- Template handling and
purification
- Focus on the
optimisation of RT-qPCR
- Various approaches to
genetic analysis
- Working with limiting
samples
- Data analysis
- Troubleshooting
- Assay improvement
option for challenging assays
- LNA technology and
application
Seminar
grading key:
- Level 1 - terrified
and beginners
- Level 2 - something
for everyone but a background knowledge is assumed
- Level 3 - improvers,
seminars focused on specific areas of interest
- Level 4 - brave and
experienced, delving into the fine detail and the more troublesome
aspects of the technique
| Available
Seminars: |
| Author |
Seminar
Title |
| Mudassir Mohammed, Ph.D. |
An Introduction to qPCR |
| Valerie Hawkes, Ph.D. |
Assay Design and
Optimisation |
| Neven Zoric, M.Sc. |
Approaches to Normal qPCR —
Facing the Challenge
of Normalization |
| Tania Nolan, Ph.D. |
Deriving a Troubleshooting
Protocol |
| Mikael Kubista, Prof. |
A Statistical Approach to
qPCR Gene Expression
Data Analysis |
| Marina Tshuikina, Ph.D. |
An Introduction to
Epigenetics Analysis by qPCR |
| Valerie Hawkes, Ph.D. |
Improving the
Discrimination and Sensitivity of
qPCR Assays using LNA |
| Chris Bass, Ph.D. |
Development of DNA-based
Diagnostic Assays for
Sensitive Screening of Mosquito Disease Vector Populations |
| Michael Pfaffl, Ph.D. |
Influence of RNA Integrity
on Real-Time RT-PCR
Quantification Data |
| Vladimir Benes |
microRNA Profiling |
| Rebecca Hands |
Technical Tips on LCM
Extraction for mRNA
Profiling |
| Jens Stolte |
cDNA Amplification with
GenomePlex® Complete
Whole Genome Amplification Kit |
| Tanya Novak, M.Sc. |
The SPUD Assay Has an
Important Role in the
Interpretation of Detecting Pneumocystis DNA in Clinical Specimens |
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Stratagene
- Fast Track
qPCR Education Program
http://www.stratagene.com/tradeshows/fasttrack.aspx |
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The Fast Track QPCR
Education Program is a comprehensive program of user training and
advanced education for users of our real-time PCR instruments. The
program provides a wide range of tools to get you up and running with
real-time PCR quickly and effectively. The program also offers you
access to expert Field Application Scientists providing advanced
education. As a leader in the training of scientists on real-time PCR,
we are proud to offer the Fast Track Education Program.
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Fast
Track QPCR Education
Program Benefits:
- Rapid
and effective QPCR training
- Advanced
application-focused education
- On-demand
educational tools
- Ongoing
education to
support new personal and evolving assays
- Consultation
with expert Field Application Scientists
Fast
Track QPCR Education
Program:
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Stratagene
Online Seminars
http://www.stratagene.com/onlineseminars/ |
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Stratagene
offers a variety of live and recorded online seminars
presented by one of our Technical Services Scientists. Simply select a
category of interest below to view the abstracts and to register for a
presentation.
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Technical
Training Courses
http://www.appliedbiosystems.com/service/training/
https://learn.appliedbiosystems.com/
https://learn.appliedbiosystems.com/realtimepcr
https://learn.appliedbiosystems.com/pcr
Basic Real-Time PCR
training EU
(2 days)
Objective:
In this course, the participant is trained on the basic knowledge of
Real-Time PCR and its applications for quantitative and SNP analysis.
With the use of practical examples, this course focuses on the correct
use of the web-based tools for searching genomic TaqMan. assays and the
SDS and the Primer Express. software. In addition, an overview of the
diverse applications of Real-Time PCR is presented.
This
training course is optimized for scientists new to ABI PRISM. Real-Time
PCR platforms and who wish to establish and optimally use the system
for Real-Time PCR and SNP analysis applications.
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