real-time qPCR workshops
qPCR Event Calendar - worldwide qPCR Events:
WorkshopsSymposia, Meetings, Conferences,  EMBO  & EMBL  events,  Seminars, qPCR Education Programs, ...

submit your qPCR event here  =>  events@gene-quantification.info


Upcoming events @  qPCR.net
  Share      previous qPCR events:

qPCR 2004   qPCR 2005   qPCR 2005 in Leipzig   qPCR 2007    qPCR 2009    qPCR 2010 in Vienna
qPCR 2007 in US    qPCR 2008 in US    qPCR 2009 in US




BioEPS is now presenting
eSeminars, eConferences & scientific talks
online via video stream!


qPCR Workshops:










      



Course 2010:

4 - 6  October 2010  (E)
3-day qPCR Basic Module (Mon. - Wed.)

7  October 2010  (E)
1-day HRM Module (Thu.)

11 - 12 October  2010  (E)
2-day qPC-R - data analysis using R packages (Mon.-Tue.)

18 - 20 October  2010  (E)
3-day single-cell & qPCR (Mon. - Wed.)

21 - 22 October  2010  (E)
2-day Experiment Design  &  qPCR data processing Module (Thu. - Fri.) 

8 - 9  November 2010  (E)
2-day microRNA & qPCR (Mon.-Tue.)

29 November - 1 December 2010  (E)
3-day qPCR Basic Module (Mon. - Wed.)

2 - 3 December 2010  (E)
2-day Experiment Design  &  qPCR data processing Module (Thu. - Fri.)


=>  more info HERE



(E) = English course
(G) = German course / deutschsprachiger Kurs

Open Courses in qPCR:

Life Science is still a growing sector and new methods and technologies are continously developed. Therefore permanent training and education becomes so important.

With our specific course program we are offering a range of high-quality course modules, in cooperation with different companies to give a general and independent overview of existing qPCR technologies and systems.
Our course issues are based on skilled know-how from own research studies and publications.

Our aim is to point out a critical way of thinking to increase the quality and outcome of experimental data.

=>  course program 2010

=>  BioEPS Flyer - eSeminars &  qPCR courses

=>  register HERE



qPCR Symposia, Meetings, and Conferences:


Thank you very much for your interest in our qPCR Symposium 2010 in Vienna!

From 7 - 9th April 330 attendee from 37 contries and 39 international companies in the industrial exhibition participate at the event.

During the symposium the presented talks and slides were recorded in high sound quality and movie resolution and they will be made available as eConfernce qPCR 2010 on our streaming server eConference.qPCR2010-Vienna.net  or   eConference.bioeps.com   

The complete qPCR 2010 eConference package
contains 50 talks in high resolution from the qPCR Symposium 2010 in Vienna with all sessions.

Or you can visit the single sessions including following keynote speakers:

All symposium participants have FREE access for a limted time to the recorded talks and to the updated online proceedings including all participants contact data.

The talks by the plenary lecturer, selected invited academic and industrial speakers will be published in METHODS special qPCR Vol 50 issue 4  (April 2010)  with the title “The ongoing evolution of qPCR”.   At the meeting all participants will get  a free hard cover of the issue => please view the TOC  and  HTML or PDF versions

Best regards

The BioEPS organisation team
Martina Reiter,  Sylvia &  Michael Pfaffl




The scientific organization is managed by international well-known scientists in the field of qPCR:

Stephen Bustin               Prof. of Molecular Science, School of Medicine, London, UK
Mikael Kubista                Prof. of Biotechnology, TATAA Biocenter, Sweden
Christine Mannhalter     Prof. for Molecular Diagnostics in Clinical Chemistry, Med. University Vienna, Austria
Jo Vandesompele          Prof. at the Center of Medical Genetics, University of Ghent, Belgium
Michael W. Pfaffl             Senior Scientist and Reader in Physiology, Technical University of Munich, Weihenstephan, Germany  (scientific coordination)

qPCR Symposium topic  - “The ongoing evolution of qPCR” - Symposium sessions:
  • MIQE and QM strategies in qPCR
    The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Following these guidelines will encourage better experimental practice, allowing more reliable and unequivocal interpretation of qPCR results. QM strategies in real-time PCR to guarantee better and more valid results.

  • High throughput quantitative PCR – digital PCR
    384 well applications, new high throughput platforms, droplet PCR, qPCR robotics, digital PCR, gene expression real-time RT-PCR arrays (mRNA and microRNA), quantitative multiplexing, …
  • HRM – High Resolution Melting - Epigentics
    SNP analysis, HRM = high resolution melt applications, Epigenetics, methylation markers, HRM platform comparison, etc …
  • CNA - Circulating Nucleic Acids
    Analysis of circulating RNAs and DNA and microRNAs as diagnostic and prognostic marker, …
  • Single-cell qPCR
    Single-cell sampling, pre-amplification techniques, laser microdissection, sub-cellular PCR, micro-manipulation of cell clusters, cellular micro injection, FACS spotting, single cell handling, pre-amplification…
  • RNAi - microRNA - siRNA Applications – miRNA normalisation
    RNAi mechanism, microRNA extraction, qRT-PCR technologies to detect microRNA, microRNA normalisation strategies, siRNA applications in combination with qRT-PCR, microRNA targets and microRNA precursors, new siRNA manipulation and microRNA technologies, ...
  • qPCR BioStatistics & BioInformatics
    software applications, data mining, calculation of relative expression, primer and probe design on mRNA and microRNA level, real-time PCR efficiency determination, mathematical modelling, multivariate expression profiling, statistics in real-time PCR, data management, multiway expression profiling, multiple regression analysis,  3D data visualization, ...



Quantifying nucleic acids by real-time PCR


A 2-day ‘hands-on’ workshop to teach the core principles of qPCR, focusing on the practical steps required to design, set-up, validate and analyse real-time qPCR assays.

Course details:  http://www.qstandard.co.uk/HTML/qPCRcourse.html

Online registration available. For queries email   info@qstandard.co.uk




3rd annual Advances in qPCR conference and exhibition

This year's event will take place in the dynamic city of Dublin, economic and cultural centre of Ireland.

The conference will be co-located with RNAi & miRNA Europe, Epigenetics Europe and Genomics Automation Europe.
Registered delegates will have access to all four meetings ensuring a very cost-effective trip.

Agenda Topics:
- qPCR in Diagnostics
- Detection of Tumour Cells
- microRNA/siRNA Applications
- High Resolution Melt
- Chip / location analysis
- Single Cell qPCR
- BioStatistics & Bioinformatics
- Expression Profiling



qPCR Symposium USA
1. - 4 November 2010
San Francisco Bay Area, Millbrae, CA ,  USA
Clarion Hotel San Fransisco Airport

www.qPCRsymposium.com

Scientific Committee Members:
  • Stephen A Bustin, Ph.D.
  • Gregory L. Shipley, Ph.D.  
  • Michael W. Pfaffl, Ph.D.   
  • Mikael Kubista Ph.D.  
  • Kevin L. Knudtson, Ph.D

  • Preanalytics – Sample preparation, extraction and purification
  • Standardization and quality control
  • High throughput expression profiling– digital PCR, integration, workflow
  • Epigenetics, mutation analysis, copy number variation
  • Molecular diagnostics of complex diseases – detection and profiling of tumor cells     
  • Single-cell and subcellular expression profiling
  • Non coding RNAs
  • Next Generation Sequencing techniques – Compliment or competitor to qPCR?
  • qPCR, Next Generation Sequencing experimental design and qPCR data mining
  • Clinical applications of qPCR and Next Generation Sequencing


                


Following MIQE Recommendations - EMBL Heidelberg, Germany
Monday 5 July - Friday 9 July 2010

Since the early descriptions of the use of quantitative Real Time PCR, the technique has been adopted in almost every aspect of life science research and is increasingly used for clinical analysis. Over time protocols and strategies have been tried and tested, amended and developed such that there are currently several different approaches. Protocol variations are evident at each step of the RT-qPCR process, from sample acquisition to data analysis (e.g. sample QC, experimental design, assay design and validation, normalisation, biostatistical interpretation, reporting, etc). It is now apparent that these adaptations may result in differences in the final biological conclusion of the study.

This workshop is based upon the MIQE guidelines. Each step of the RT-qPCR process will be discussed and protocol variations illustrated practically. The student will be instructed in best practice and acceptable alternative strategies. EMBL Corporate Partnership Registration Fee Fellowships available for this meeting.

http://www.embl.de/training/courses_conferences/course/2010/MIQ10-01/index.html



qPCR Seminars and Education Programs:



Location

Bio EPSBioEPS GmbH,
Lise-Meitner-Strasse 30
85354 Freising
Germany

Overview

Real-time or quantitative PCR (qPCR) allows quantification of starting amounts of DNA, cDNA, or RNA templates. qPCR is based on the detection of a fluorescent reporter molecule that increases as PCR product accumulates with each cycle of amplification. Fluorescent reporter molecules include dyes that bind double-stranded DNA (i.e. SYBR Green I) or sequence-specific probes (i.e. Molecular Beacons or TaqMan® Probes). qPCR exceeds the limitations of traditional end-point PCR methods by allowing either absolute or relative quantification of the PCR product at the end of each cycle. This qPCR Workshop is an introductory course with theoretical and practical sessions. Participants will get an overview of qPCR technology from sampling to data analysis. Hands-on experiments show how to perform qPCR experiments under the supervision of Dr. Martina Reiter and Dr. Michael Pfaffl, both experts in qPCR technology and data analysis. Sessions on data analysis, normalization and quantification methods will explain how to reliable interpret qPCR results.

Registration Form








Free of charge web seminars

Sigma-Aldrich is pleased to be able to bring you a series of webex seminars dedicated to qPCR. They will include technical presentations from international speakers discussing the latest techniques and aspects of qPCR. To register, go to QPCR Seminars
Sigma-Aldrich has the pleasure of inviting you to a seminar series devoted to the technical aspects of qPCR and RT-qPCR.

Expert speakers will cover areas such as:
  • QPCR assay design and optimisation
  • Template handling and purification
  • Focus on the optimisation of RT-qPCR
  • Various approaches to genetic analysis
  • Working with limiting samples
  • Data analysis
  • Troubleshooting
  • Assay improvement option for challenging assays
  • LNA technology and application
Seminar grading key:
  • Level 1 - terrified and beginners
  • Level 2 - something for everyone but a background knowledge is assumed
  • Level 3 - improvers, seminars focused on specific areas of interest
  • Level 4 - brave and experienced, delving into the fine detail and the more troublesome aspects of the technique
Available Seminars:
Author Seminar Title
Mudassir Mohammed, Ph.D. An Introduction to qPCR
Valerie Hawkes, Ph.D. Assay Design and Optimisation
Neven Zoric, M.Sc. Approaches to Normal qPCR — Facing the Challenge of Normalization
Tania Nolan, Ph.D. Deriving a Troubleshooting Protocol
Mikael Kubista, Prof. A Statistical Approach to qPCR Gene Expression Data Analysis
Marina Tshuikina, Ph.D. An Introduction to Epigenetics Analysis by qPCR
Valerie Hawkes, Ph.D. Improving the Discrimination and Sensitivity of qPCR Assays using LNA
Chris Bass, Ph.D. Development of DNA-based Diagnostic Assays for Sensitive Screening of Mosquito Disease Vector Populations
Michael Pfaffl, Ph.D. Influence of RNA Integrity on Real-Time RT-PCR Quantification Data
Vladimir Benes microRNA Profiling
Rebecca Hands Technical Tips on LCM Extraction for mRNA Profiling
Jens Stolte cDNA Amplification with GenomePlex® Complete Whole Genome Amplification Kit
Tanya Novak, M.Sc. The SPUD Assay Has an Important Role in the Interpretation of Detecting Pneumocystis DNA in Clinical Specimens


Stratagene - Fast Track qPCR Education Program

http://www.stratagene.com/tradeshows/fasttrack.aspx



The Fast Track QPCR Education Program is a comprehensive program of user training and advanced education for users of our real-time PCR instruments. The program provides a wide range of tools to get you up and running with real-time PCR quickly and effectively. The program also offers you access to expert Field Application Scientists providing advanced education. As a leader in the training of scientists on real-time PCR, we are proud to offer the Fast Track Education Program.

Fast Track QPCR Education Program Benefits:

  • Rapid and effective QPCR training
  • Advanced application-focused education
  • On-demand educational tools
  • Ongoing education to support new personal and evolving assays
  • Consultation with expert Field Application Scientists

Fast Track QPCR Education Program:


Stratagene Online Seminars

http://www.stratagene.com/onlineseminars/
Register for and participate in LIVE online seminars presented in real-time by one of our Technical Services scientists. Also available are online tutorials and software demonstrations.
Stratagene offers a variety of live and recorded online seminars presented by one of our Technical Services Scientists. Simply select a category of interest below to view the abstracts and to register for a presentation.
Microarray Reagents PCR Reagents QPCR   QPCR Extended Web Modules Viral Delivery Systems




Technical Training Courses

http://www.appliedbiosystems.com/service/training/

https://learn.appliedbiosystems.com/

https://learn.appliedbiosystems.com/realtimepcr

https://learn.appliedbiosystems.com/pcr

Basic Real-Time PCR training EU (2 days)

Objective:
In this course, the participant is trained on the basic knowledge of Real-Time PCR and its applications for quantitative and SNP analysis. With the use of practical examples, this course focuses on the correct use of the web-based tools for searching genomic TaqMan. assays and the SDS and the Primer Express. software. In addition, an overview of the diverse applications of Real-Time PCR is presented.

This training course is optimized for scientists new to ABI PRISM. Real-Time PCR platforms and who wish to establish and optimally use the system for Real-Time PCR and SNP analysis applications.

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