qPCR Workshops:
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Course 2010:
4
- 6
October 2010
(E)
3-day
qPCR Basic Module (Mon.
- Wed.)
7
October 2010
(E)
1-day HRM
Module (Thu.)
11 - 12 October
2010 (E)
2-day qPC-R - data
analysis using R packages (Mon.-Tue.)
18 - 20 October
2010 (E)
3-day
single-cell &
qPCR (Mon. -
Wed.)
21 - 22 October
2010 (E)
2-day Experiment
Design &
qPCR data processing
Module (Thu. -
Fri.)
8 - 9 November 2010 (E)
2-day microRNA & qPCR
(Mon.-Tue.)
29 November - 1 December 2010 (E)
3-day qPCR
Basic Module (Mon.
- Wed.)
2 -
3 December 2010 (E)
2-day Experiment
Design & qPCR data processing
Module (Thu. -
Fri.)
=>
more info HERE
(E)
= English course
(G)
= German course /
deutschsprachiger Kurs
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Open
Courses in
qPCR:
Life Science is
still a growing sector and new methods and technologies are continously
developed. Therefore permanent training and education becomes so
important.
With our specific course program we are offering a range of
high-quality course modules, in cooperation with different companies to
give a general and independent overview of existing qPCR technologies
and systems.
Our course issues are based on skilled know-how from own research
studies and publications.
Our aim is to point out a critical way of thinking to increase the
quality and outcome of experimental data.
=> course
program 2010
=> BioEPS Flyer - eSeminars
& qPCR courses
=>
register HERE |
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qPCR Symposia, Meetings, and
Conferences:
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Dear researcher,
The
scientific organization is managed by international well-known
scientists in the field of qPCR:
Stephen
Bustin
Prof. of Molecular Science, School
of Medicine, London, UK
Mikael
Kubista
Prof. of Biotechnology, TATAA Biocenter, Sweden
Christine Mannhalter Prof. for Molecular
Diagnostics in Clinical Chemistry, Med. University Vienna, Austria
Jo Vandesompele
Prof.
at the Center of Medical Genetics, University of Ghent, Belgium
Michael W.
Pfaffl
Senior Scientist and Reader in Physiology, Technical University of
Munich, Weihenstephan, Germany (scientific coordination)
qPCR
Symposium
topic - “The ongoing evolution
of qPCR” - Symposium
sessions:
- MIQE
and QM strategies in qPCR
The MIQE guidelines: minimum information for
publication of
quantitative real-time PCR experiments. Following these guidelines will
encourage better experimental practice, allowing more reliable and
unequivocal interpretation of qPCR results. QM strategies in real-time
PCR to guarantee better and more valid results.
- High
throughput quantitative PCR
– digital PCR
384 well applications, new high throughput
platforms, droplet PCR, qPCR
robotics, digital PCR, gene expression real-time RT-PCR arrays (mRNA
and microRNA), quantitative multiplexing, …
- HRM
–
High Resolution Melting - Epigentics
SNP analysis, HRM = high resolution melt
applications, Epigenetics,
methylation markers, HRM platform comparison, etc …
- CNA
- Circulating Nucleic Acids
Analysis of circulating RNAs and DNA and
microRNAs as diagnostic and
prognostic marker, …
- Single-cell
qPCR
Single-cell sampling, pre-amplification
techniques, laser
microdissection, sub-cellular PCR, micro-manipulation of cell clusters,
cellular micro injection, FACS spotting, single cell handling,
pre-amplification…
- RNAi
-
microRNA - siRNA
Applications – miRNA normalisation
RNAi mechanism, microRNA extraction, qRT-PCR
technologies to detect
microRNA, microRNA normalisation strategies, siRNA applications in
combination with qRT-PCR, microRNA targets and microRNA precursors, new
siRNA manipulation and microRNA technologies, ...
- qPCR
BioStatistics &
BioInformatics
software applications, data mining, calculation
of relative expression,
primer and probe design on mRNA and microRNA level, real-time PCR
efficiency determination, mathematical modelling, multivariate
expression profiling, statistics in real-time PCR, data management,
multiway expression profiling, multiple regression analysis, 3D
data visualization, ...
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Quantifying nucleic acids by real-time PCR
A 2-day ‘hands-on’ workshop to teach the core principles of qPCR,
focusing on the practical steps required to design, set-up, validate
and analyse real-time qPCR assays.
Course details: http://www.qstandard.co.uk/HTML/qPCRcourse.html
Online registration available. For queries email info@qstandard.co.uk
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3rd annual Advances in qPCR conference and
exhibition
This year's event will take place in the dynamic city of Dublin,
economic and cultural centre of Ireland.
The conference will be co-located with RNAi & miRNA Europe,
Epigenetics Europe and Genomics Automation Europe.
Registered delegates will have access to all four meetings ensuring a
very cost-effective trip.
Agenda Topics:
- qPCR in Diagnostics
- Detection of Tumour Cells
- microRNA/siRNA Applications
- High Resolution Melt
- Chip / location analysis
- Single Cell qPCR
- BioStatistics & Bioinformatics
- Expression Profiling
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qPCR
Symposium USA
1. - 4
November 2010
San Francisco Bay Area, Millbrae, CA ,
USA
Clarion Hotel San Fransisco Airport
www.qPCRsymposium.com |
Scientific
Committee Members:
- Stephen A Bustin,
Ph.D.
- Gregory L. Shipley,
Ph.D.
- Michael W. Pfaffl,
Ph.D.
- Mikael Kubista Ph.D.
- Kevin L. Knudtson, Ph.D
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- Preanalytics – Sample preparation,
extraction and purification
- Standardization and quality control
- High throughput expression profiling–
digital PCR, integration, workflow
- Epigenetics, mutation analysis, copy
number variation
- Molecular diagnostics of complex
diseases – detection and profiling of tumor
cells
- Single-cell and subcellular expression
profiling
- Non coding RNAs
- Next Generation Sequencing techniques –
Compliment or competitor to qPCR?
- qPCR, Next Generation Sequencing
experimental design and qPCR data mining
- Clinical applications of qPCR and Next
Generation Sequencing
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Following MIQE Recommendations - EMBL
Heidelberg, Germany
Monday 5 July - Friday 9 July 2010
Since the early descriptions of the use of quantitative Real Time PCR,
the technique has been adopted in almost every aspect of life science
research and is increasingly used for clinical analysis. Over time
protocols and strategies have been tried and tested, amended and
developed such that there are currently several different approaches.
Protocol variations are evident at each step of the RT-qPCR process,
from sample acquisition to data analysis (e.g. sample QC, experimental
design, assay design and validation, normalisation, biostatistical
interpretation, reporting, etc). It is now apparent that these
adaptations may result in differences in the final biological
conclusion of the study.
This workshop is based upon the MIQE guidelines. Each step of the
RT-qPCR process will be discussed and protocol variations illustrated
practically. The student will be instructed in best practice and
acceptable alternative strategies. EMBL Corporate Partnership
Registration Fee Fellowships available for this meeting.
http://www.embl.de/training/courses_conferences/course/2010/MIQ10-01/index.html
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qPCR
Seminars and Education Programs:
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Location
Bio EPSBioEPS GmbH,
Lise-Meitner-Strasse 30
85354 Freising
Germany
Overview
Real-time or quantitative PCR (qPCR) allows quantification of starting
amounts of DNA, cDNA, or RNA templates. qPCR is based on the detection
of a fluorescent reporter molecule that increases as PCR product
accumulates with each cycle of amplification. Fluorescent reporter
molecules include dyes that bind double-stranded DNA (i.e. SYBR Green
I) or sequence-specific probes (i.e. Molecular Beacons or TaqMan®
Probes). qPCR exceeds the limitations of traditional end-point PCR
methods by allowing either absolute or relative quantification of the
PCR product at the end of each cycle. This qPCR Workshop is an
introductory course with theoretical and practical sessions.
Participants will get an overview of qPCR technology from sampling to
data analysis. Hands-on experiments show how to perform qPCR
experiments under the supervision of Dr. Martina Reiter and Dr. Michael
Pfaffl, both experts in qPCR technology and data analysis. Sessions on
data analysis, normalization and quantification methods will explain
how to reliable interpret qPCR results.
Registration
Form
Free
of charge web
seminars
Sigma-Aldrich
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
presentations
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
Sigma-Aldrich
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
Expert
speakers
will cover areas such as:
- QPCR assay design and
optimisation
- Template handling and
purification
- Focus on the
optimisation of RT-qPCR
- Various approaches to
genetic analysis
- Working with limiting
samples
- Data analysis
- Troubleshooting
- Assay improvement
option for challenging assays
- LNA technology and
application
Seminar
grading key:
- Level 1 - terrified
and beginners
- Level 2 - something
for everyone but a background knowledge is assumed
- Level 3 - improvers,
seminars focused on specific areas of interest
- Level 4 - brave and
experienced, delving into the fine detail and the more troublesome
aspects of the technique
| Available
Seminars: |
| Author |
Seminar
Title |
| Mudassir Mohammed, Ph.D. |
An Introduction to qPCR |
| Valerie Hawkes, Ph.D. |
Assay Design and
Optimisation |
| Neven Zoric, M.Sc. |
Approaches to Normal qPCR —
Facing the Challenge
of Normalization |
| Tania Nolan, Ph.D. |
Deriving a Troubleshooting
Protocol |
| Mikael Kubista, Prof. |
A Statistical Approach to
qPCR Gene Expression
Data Analysis |
| Marina Tshuikina, Ph.D. |
An Introduction to
Epigenetics Analysis by qPCR |
| Valerie Hawkes, Ph.D. |
Improving the
Discrimination and Sensitivity of
qPCR Assays using LNA |
| Chris Bass, Ph.D. |
Development of DNA-based
Diagnostic Assays for
Sensitive Screening of Mosquito Disease Vector Populations |
| Michael Pfaffl, Ph.D. |
Influence of RNA Integrity
on Real-Time RT-PCR
Quantification Data |
| Vladimir Benes |
microRNA Profiling |
| Rebecca Hands |
Technical Tips on LCM
Extraction for mRNA
Profiling |
| Jens Stolte |
cDNA Amplification with
GenomePlex® Complete
Whole Genome Amplification Kit |
| Tanya Novak, M.Sc. |
The SPUD Assay Has an
Important Role in the
Interpretation of Detecting Pneumocystis DNA in Clinical Specimens |
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Stratagene
- Fast Track
qPCR Education Program
http://www.stratagene.com/tradeshows/fasttrack.aspx |
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The Fast Track QPCR
Education Program is a comprehensive program of user training and
advanced education for users of our real-time PCR instruments. The
program provides a wide range of tools to get you up and running with
real-time PCR quickly and effectively. The program also offers you
access to expert Field Application Scientists providing advanced
education. As a leader in the training of scientists on real-time PCR,
we are proud to offer the Fast Track Education Program.
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Fast
Track QPCR Education
Program Benefits:
- Rapid
and effective QPCR training
- Advanced
application-focused education
- On-demand
educational tools
- Ongoing
education to
support new personal and evolving assays
- Consultation
with expert Field Application Scientists
Fast
Track QPCR Education
Program:
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Stratagene
Online Seminars
http://www.stratagene.com/onlineseminars/ |
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Stratagene
offers a variety of live and recorded online seminars
presented by one of our Technical Services Scientists. Simply select a
category of interest below to view the abstracts and to register for a
presentation.
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Technical
Training Courses
http://www.appliedbiosystems.com/service/training/
https://learn.appliedbiosystems.com/
https://learn.appliedbiosystems.com/realtimepcr
https://learn.appliedbiosystems.com/pcr
Basic Real-Time PCR
training EU
(2 days)
Objective:
In this course, the participant is trained on the basic knowledge of
Real-Time PCR and its applications for quantitative and SNP analysis.
With the use of practical examples, this course focuses on the correct
use of the web-based tools for searching genomic TaqMan. assays and the
SDS and the Primer Express. software. In addition, an overview of the
diverse applications of Real-Time PCR is presented.
This
training course is optimized for scientists new to ABI PRISM. Real-Time
PCR platforms and who wish to establish and optimally use the system
for Real-Time PCR and SNP analysis applications.
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