With this page and
all the presented tools we will help you with to find the right
information about qPCR and related topics in Molecular Biology in the
literature and in the World Wide Web => qPCR
Directory: A-Z
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=>
Biocompare
Product List - PCR
NEW !
=>
Biocompare
Product List - real-time PCR
NEW !
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qPCR basics
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What
can I do with qPCR?
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Genome Technology Tech Guides Listing
Genotyping/PGx
- Instrument Guide - Clinical Genotyping
- Tech Guide - SNP Genotyping
- Tech Guide - Clinical Genotyping
- Validating Cancer Biomarkers Tech Guide
- Genotyping Tech Guide
Microarrays
- Tech Guide - cDNA Microarrays
- Tech Guide - Microarray Sample Prep
- Tech Guide - Microarray Confirmation
& Quality Control
- Tech Guide - Microarray Analysis:
Genome-wide Association
- ChIP-chip Tech Guide
- Array CGH Tech Guide
Proteomics
- Tech Guide - Mass Spec Sample Prep
- Tech Guide - PhosphoProteomics-PTM
- Tech Guide - Protein-Protein
Interactions
- Tech Guide - High-Throughput
Chromatography
- Tech Guide - Protein Microarrays
- Tech Guide - MALDI-TOF
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PCR
- Tech Guide - Real Time PCR Volume I
- Tech Guide - Real Time PCR Volume II
- Tech Guide - Real Time PCR Volume III
- Tech Guide - Clinical qPCR (Volume IV)
- Tech Guide - Real Time PCR for
microRNAs
- Best Practices Guide - Sample Prep
- Real-time PCR Tech Guide
- Real-time PCR Tech Guide, vol VII
- Real-time PCR Tech Guide, vol VIII
RNA Interference
- Tech Guide - siRNA Design
- Tech Guide - shRNA Design
Sample Prep
- DNA Extraction and FFPE Tech Guide
- Agbio Sample Prep Tech Guide
Sequencing
- Tech Guide - Sequencing Sample Prep
- Tech Guide - DNA Sequencing
- Tech Guide - Sequence Alignment &
Assembly
- Assembly/Alignment for Next-Gen
Sequencing Tech Guide
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BioMedSearch is an enhanced version of the NIH PubMed
search that combines MedLine/PubMed data with data from other sources
to make the most comprehensive biomedical literature search available.
BioMedSearch also provides advanced account features that allow saved
searches, alerts, saving documents to portfolios, commenting on
documents and portfolios, and sharing documents with other registered
users. Registering for BioMedSearch is free => http://www.biomedsearch.com/
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Scitable is a Social
Network for Science
If you have an interest in science, or specifically in genetics, you
can always turn to scientific journals and publications for
information. But if you're looking for reference material or an
opportunity to connect with researchers and scientists in the field, Scitable can
help.
Gene Expression and
Regulation Lead Editor: Laura Hoopes |
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Scitable
is billed as a learning and research tool for people
interested in genetics, but under the hood it's much more than just a
reference utility. The service offers you the ability to connect with
researchers and scientists around the globe and influence the
scientific discussion around genetics while also giving you a way to
learn more about it.
The first thing you'll want to do at Scitable is sign up for an
account. They're free, and give you access to all of the site's
features, including the ability to create a profile for yourself,
contribute articles and multimedia to the community, and access to the
site's over 150 free articles and peer-reviewed papers on topics from
genetic engineering to evolutionary biology. The service is aimed
primarily at teachers, professors, and their students, but if you have
an interest in science, biology, or genetics, don't hesitate to sign up. |
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What's
new at GoPubMed ... ?
- The first major change is
that MeSHPubmed is now included in GoPubMed. Gene Ontology (GO) and
Medical Subject Headings (MeSH) are now integrated into one
application. This change was requested by many users and enables the
joint search in molecular biology and medicine!
- Novel award winning text
mining method for automated recognition of protein names. Proteins are
linked to the UniProt database.
- Using the clipboard
functionality the user can collect selected articles from one or more
searches and export them to Endnote, BibTeX, Plain text, or XML formats.
- New export functionalities
for each citation or the first 100 search results.
- Toggle between abstract and
snippet view.
- HotTopics, an
alternative way to get an overview on biomedical literature over the
last years, is automatically linked to your query.
- A complete new
interface. Like it? Drop us a comment: help@transinsight.com ... we
appreciate!
How
Does It Work?
When people search, they have questions in mind.
GoPubMed allows to significantly faster find information needed through
the use of background knowledge. => GoPubMed
- retrieves PubMed abstracts
for your search query,
- detects terms from the Gene
Ontology (GO) and Medical Subject Headings (MeSH) in the abstracts,
- displays the subset of the
GO and MeSH relevant to the keywords, and
- allows you to browse
the ontologies and display only papers containing specific GO and MeSH
terms.
After
performing a search, the resulting abstracts are annotated with
your query keywords and GO and MeSH terms. The abstracts are grouped
using the GO and MeSH terms, which appear in the text. Now the GO and
MeSH hierarchies can be used to systematically explore the search
results.
Note that only a subset of all GO and MeSH terms may be relevant to
your query. This subset – more frequent terms - is presented on the
left hand side. Sorting documents to a highly organised network
facilitates the finding of relevant documents significantly.
The hierarchy of content shows the whole GO and MeSH ontologies. GO and
MeSH serve as table of contents in order to structure the over 16
million articles of the MEDLINE data base.
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New
search functions on Google Scholar !
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about GOOGLE
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Google
Scholar enables you to search specifically for scholarly
literature, including peer-reviewed
papers, theses, books, pre-prints, abstracts and technical reports from
all broad areas of research. Use Google Scholar to find articles from a
wide variety of academic publishers, professional societies, preprint
repositories and universities, as well as scholarly articles available
across the web.
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Google Scholar:
The New Generation of Citation Indexes
by ALIREZA NORUZ, Libri 2005
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Google Scholar (http://scholar.google.com)
provides a new method of locating potentially relevant articles on a
given subject by identifying subsequent articles that cite a previously
published article. An important feature of Google Scholar is that
researchers can use it to trace interconnections among authors citing
articles on the same topic and to determine the frequency with which
others cite a specific article, as it has a "cited by" feature. This
study begins with an overview of how to use Google Scholar for citation
analysis and identifies advanced search techniques not well documented
by Google Scholar. This study also compares the citation counts
provided by Web of Science and Google Scholar for articles in the field
of "Webometrics." It makes several suggestions for improving Google
Scholar. Finally, it concludes that Google Scholar provides a free
alternative or complement to other citation indexes.
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Can't find the
right info or publication
on my sites ?
- Summary of Biology glossaries and Biology dictionaries.
- Summary of Live Science glossaries and Life Science dictionaries.
- Summary of Genetics glossaries and Genetics dictionaries.
- MedBio
World:
MedBioWorld
with over 35,000 links, is
one of the largest medical and bioscience resource portal and directory
on the Internet. Medical and bioscience professionals have access to
resources that include timely medical and bioscience news; extensive
journal, association and database listings; professional conference
coverage; continuing medical education activities; access to full-text
medical journal articles; and drug and medical literature databases.
This portal is ranked 1st for scientific journals, bioscience journals
and bioscience directories and 2nd for medical journals by Google. The
site provides access to:
9,763
Journals
3,079 Databases
8,677
Associations
2,092 Bioscience Companies
- Kimball's
Biology Pages Online-Glossar der Biologie
- Online
Medical & Science Dictionaries Lists of online science and medical
dictionaries and glossaries
- BioTech
Science Dictionary
(University of Indiana) provides an illustrated glossary containing
terms associated with genetics and biochemistry; the dictionary also
includes some terms associated with biology, chemistry,
pharmacology, toxicology & medicine.
- The
Cell: A Molecular Approach - Glossary
- BioABACUS
a searchable database of abbreviations and acronyms in Biotechnology
that contains terms in such categories
as: Biochemistry, Cell Biology u.a.
- The
Dictionary of Cell Biology
(Julian Dow, Glasgow University) contains thousands of cell biology
terms and cross-references; a new graphical interface facilitates
browsing of topic areas.
- A Web of
On-line Dictionaries
(Bucknell University) is linked to over 600 dictionaries in 150
languages; the site also has a convenient Quick Look-Up function.
- WWWebster Dictionary provides
easy access
to the Merriam Webster dictionary and thesaurus.
- GLOSSARY
OF REAL-TIME PCR TERMS by M.Tevfik Dorak
- Molecular Biology Glossary
- A Hypermedia Glossary of Genetic Terms pick from
an alphabetical List of Terms with 670 entries
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BioTechniques®
Molecular Biology Techniques
Forums
Welcome
to the BioTechniques® Molecular Biology
Forums, a
science-based bulletin board for techniques, tips, and questions
concerning molecular biology, cell biology, microscopy, and
bioinformatics.
MolecularBiology.forums.biotechniques.com |
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qPCRforum.com : Discuss GenEx and qPCR
Welcome to qPCRforum - Forum for discussion about GenEx and qPCR
(quantitative real-time PCR) |
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http://www.bioinformatics.vg/forums/
Subscribe to the
Bioinformatics.Net Newsletter featuring the new "Bioinformatics
from a User's Perspective" series by submitting your email address
below. On average you will receive no more than one newsletter each
month, and
we will not share your email address with 3rd parties. You can
unsubscribe
at any time. View previous
newsletters
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Multidisciplinary Discussion and Debate Forum
Description:
ScienceChatForum (SCF) is a discussion medium, and as such its primary
purpose is the forum of discussion for science and related matters. |
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Scientist
Solutions - Your International Life Science
Form
By Scientists.....for Scientists
The
ScientistSolutions board provides a discussion forum for professionals
in the life sciences, where researchers can ask and answer questions,
exchange protocols, and post jobs. www.scientistsolutions.com |
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- Upload
your own lab protocols for
further feedback and refinement
- Comment on
published Nature Protocols, or
protocols submitted by other researchers
- Discuss
techniques in our
discussion forum
- Read
latest protocols on the network
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http://www.protocol-online.org/
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BioTechniques' Protocol Guide Offers
Solutions for Science.
BioTechniques®'
Protocol Guide is a collection of detailed, optimized
procedures/protocols for use in the lab. Organized by category,
BioTechniques' Protocol Guide contains specific applications of
commercial products in a wide range of areas. Even better, protocols
are updated quarterly to provide you with the very latest product
information.
With
BioTechniques®' Protocol Guide, you have access to detailed product
information in a downloadable PDF format-which can be easily added to a
notebook for ready access. Each protocol is accompanied by a link to
the company web site and an e-mail link to contact the company directly.
http://www.biotechniques.com/default.asp?page=protocol
High Resolution Melt (HRM) by
BioTechniques Protocol Guide: p 67 (Dec 2006)
view protocol
Complete Real-Time PCR Solutions
BioTechniques Protocol Guide: p 71 (Dec 2006)
view protocol
Normalization
Methods for qPCR by
BioTechniques Protocol Guide:
p 63 (Nov 2007) view protocol
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BioProtocols
- Protocols contributed by scientists from
over 125 academic laboratories
- World renowned Editorial Board
- Wide variety of life science disciplines
covered
- Searchable protocol database
- Printable versions available for all
protocols
- Store protocols of particular interest to
you and send protocols to your colleagues using the My Protocols feature
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Nucleic Acids
Research Methods
Each of the collections on this page is a category-specific archive of
Methods published in NAR from 1999 to the present. The numbers in
parentheses show the number of articles currently in each collection.
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| PCR books |
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A list of useful and available books on Polymerase Chain Reaction
Technique since 1990 up to now as
well as PCR Books that will be published in 2003. This section contain
74 links with Title, Publication year, and Edition number to vendors of
these books as well as additional information. PCR Books are sorted by
publishing year, and then alphabetically. http://www.pcrlinks.com/resources/books1.htm
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PCR related books available on Amazon
http://www.amazon.com
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some full text articles are in Adobe
Acrobat Format (
PDF ) 
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Real-Time
PCR: Current Technology and Applications
An important new work on "Real-Time PCR" is
planned for release later this year. Bringing together the expertise
and enthusiasm of a panel of leading researchers the book constitutes
an essential manual on the latest technology and applications of real
time PCR. Full details at http://www.horizonpress.com/realtimepcr
Publisher: Caister Academic Press
Edited by: Julie Logan, Kirstin Edwards and
Nick Saunders
Publication date: January 2009 (initial books
available autumn 2008, order now!)
ISBN: 978-1-904455-39-4
Topics
include:
- An Introduction to Real-Time PCR
- An Overview of PCR Platforms
- Homogeneous Fluorescent Chemistries for
Real-Time PCR
- Reference Gene Validation Software for
Improved Normalization
- Data Analysis Software
- Performing Real-time PCR
- Internal and External Controls for Reagent
Validation
- Introduction to the Applications of
Real-Time PCR
- Analysis of mRNA Expression by Real-Time PCR
- Validation of Array Data
- Mutation Detection by Real-Time PCR
- Real-Time NASBA
- Applications in Clinical Microbiology
- Diagnosis of Invasive Fungal Infections
- Biodefense
- Real-Time PCR: Application to Food
Authenticity and Legislation
- Molecular Haplotyping by Real-time PCR
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A-Z
of quantitative PCR ( Editor: S.A. Bustin )
International
University Line
(IUL), La Jolla, CA, USA
Flyer for the book
=> PDF
Search
inside @ Amazon.com
http://www.amazon.com/gp/reader/0963681788/ref=sib_dp_pt/102-2200945-3924931#reader-link
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Real Time
PCR (Advanced Methods)
by
Tevfik Dorak
http://www.amazon.com
Real-time PCR is
based on the
conventional principles of PCR and, with the simple shift of emphasis
from the end-product to the whole course of the PCR process, has
established itself as the most sensitive and specific quantitative PCR
method. Real-time PCR is, like any other modern method in molecular
genetics, expanding, with potential applications even in proteomics.
With
a variety of detection chemistries, an increasing number of platforms,
multiple choices for analytical methods and the jargon emerging along
with these developments, real-time PCR is facing the risk of becoming
an intimidating method, especially for beginners. Real-Time PCR
provides the basics, explains how they are exploited to run a real-time
PCR assay, how the assays are run and where these assays are
informative in real life. It doesn't cover every aspect of real-time
PCR, but addresses the most practical aspects of the techniques with
the emphasis on 'how to do it in the laboratory'. Keeping with the
spirit of the Advanced Methods Series, most chapters provide an
experimental protocol as an example of a specific assay.
The
BIOS Advanced Methods series is intended for advanced undergraduates,
graduate students and established research scientists. Titles in this
series are designed to cover current important areas of research in
life sciences, and include both theoretical background and detailed
protocols. The aim is to give researchers sufficient theory, supported
by references, to take the given protocols and adapt them to their
particular experimental systems.
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DNA Amplification:
Current
Technologies and Applications
http://www.horizonpress.com/hsp/abs/absdna.html
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European Federation of Biotechnology
Timeline of
Biotechnology |
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Real-Time PCR: An Essential Guide
Publisher:
Horizon Bioscience
Editor: Kirstin Edwards, Julie Logan and Nick Saunders
Genomics Proteomics and Bioinformatics Unit, Health Protection Agency,
London
qpcr-book.pdf |
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PCR Troubleshooting and Optimization - The
Essentail Guide
edited by Suzanne Kennedy & Nick Oswald
Chapter 5 - RT-PCR Optimisation Strategies
by Martina Reiter & Michael W. Pfaffl
Physiology Weihenstephan, Weihenstephaner Berg 3, 85354 Freising,
Germany
PCR technology is
based on a simple principle; an enzymatic reaction that increases the
initial amount of nucleic acids. This method makes it possible to
detect specific mRNA transcripts in any biological sample. Performing
RT-PCR analysis does not only comprehend this experimental PCR step.
Following the whole workflow of a RT-PCR quantitative analysis, it
starts with the sampling step, followed by nucleic acid extraction and
stabilization, cDNA synthesis and finally the qPCR where the mRNA
quantification takes place. Problems arise when optimization of the
experimental work flow becomes necessary because of high technical
variations. The PCR reaction itself is a quite stable reaction with
reproducibility between 2-8%. Therefore the source of experimental
variances can often be found in the pre-PCR analytical steps. Usually
this is neglected and optimization is done for PCR reaction only. In
this chapter – RT-PCR optimization strategies - the whole workflow of
RT-PCR experiment will be discussed, because the identification of the
source of variability is only possible following error accumulation in
every single step. Reliable data can be created when the technical
variance caused by the experimental steps is kept as low as possible.
In this chapter many recommendations to decrease the technical variance
can be found. |
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Nucleic
Acids Research Methods
Each of the collections on this page is a category-specific archive of
Methods published in NAR from 1999 to the present. The numbers
in parentheses show the number of articles currently in each collection.
http://nar.oxfordjournals.org/collections/
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The
Cell: A Molecular Approach
Fourth Edition
by Geoffrey M. Cooper and Robert E. Hausman;
Published by ASM Press and Sinauer Associates
http://www.sinauer.com/cooper/4e/index.html
This companion website is designed to help
students using The Cell
master the range of material presented in the textbook. The site
provides you with resources for learning the key structures, processes,
and terms of cell biology, as well as assessments to gauge your
comprehension. (Note that the quizzes and problems may be available to
you on a purely self-review basis, or they may be assigned by your
instructor. Please check with your instructor for details.)
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MIT Biology Hypertextbook
Description:
A website which contains the basic molecular biology that is the basis
of MIT's core Biology course, "Introductory Biology"
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A Biology Hypertextbook
Description:
Presented items are modified lecture outlines the author have developed
over the many years of teaching college-level introductory biology.
Excellent for students and education purposes. Rich in nice images
collected over the years.
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A Molecular Biology Notebook
Description:
Educational and research resource for students and biologists. Simple
and clear tutorials on the cell, the DNA, Molecular Engineering and
much more.
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BioDidac
Description: A bank of digital resources for
teaching biology. Great educational site.
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Biology in Motion
Description:
Here you will find animations, interactive activities, and cartoons
designed to make learning biology a rich and engaging process.
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The Molecules of Life
Description:
A school-level interactive resource by Craig D. Livingstone and Martin
E. Noble, with photographs, schemes and even movies illustrating
clearly and shortly the main techniques used in a
biophisics-biochemistry lab "to gain understanding on the structure of
protein molecules".
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http://www.web-books.com/MoBio/
Chapter
1 - Cells
and Viruses - Overview
Chapter
2 - Protein Structure and Function
Chapter
3 - Nucleic Acids, Genomics and Proteomics
Chapter
4 - Gene Transcription
Chapter
5 - Posttranscriptional Processes
Chapter
6 - Cell Signaling and Apoptosis
Chapter
7 - DNA Replication, Mutation and Repair
Chapter
8 - Cell Division and DNA Recombination
Chapter
9 - Biotechnology and Bioinformatics
Chapter
10 - Genes and Disease
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