Reference genes / Housekeeping genes
Normalization
in real-time PCR - page 4
Normalization in real-time PCR - page 1
Normalization in real-time PCR - page 2
Normalization in real-time PCR - page 3


Tools:


New selected normalisation papers:
Universal reference method for real-time.
Neil Ivan Bower, Ralf Joachim Moser, Jonathan Robert Hill, and Sigrid Arabella Lehnert
BioTechniques (2007) 42: 199-206

Validation of rat reference genes for improved quantitative gene expression analysis using low density arrays.
Jenny Hong Cai, Shibing Deng, Steven W. Kumpf, Patricia A. Lee, Panayiotis Zagouras, Anne Ryan, and Dan S. Gallagher
BioTechniques (2007) 42: 503-512

In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR.
Monika Jung, Azizbek Ramankulov, Jan Roigas, Manfred Johannsen, Martin Ringsdorf, Glen Kristiansen & Klaus Jung
BMC Molecular Biology 2007, 8:47

GeneChip, geNorm, and gastrointestinal tumors: novel reference genes for real-time PCR.
Mark Kidd,1 Boaz Nadler,2 Shrikant Mane,3 Geeta Eick,1 Maximillian Malfertheiner,1 Manish Champaneria,1 Roswitha Pfragner,4 and Irvin Modlin1
1Gastrointestinal Research Group and 3Keck Laboratory, Yale University School of Medicine, New Haven, Connecticut;
2Department of Computer Science and Applied Mathematics, Weizmann Institute of Science, Rehovot, Israel and  4Institute
of Pathophysiology, Centre for Molecular Medicine, Medical University of Graz, Austria
Physiol Genomics (2007) 30:  363–370

Developmental expression profiles of Xenopus laevis reference genes.
Sindelka R, Ferjentsik Z, Jonak J.
Dev Dyn. 2006 235(3):754-8.


Statistical Selection of Maintenance Genes for Normalization of Gene Expressions.
Yifan Huang Jason C. Hsu† Mario Peruggia‡ Abigail A. Scott
Statistical Applications in Genetics and Molecular Biology (2006) 5(1)1 Article 4

Quantification of cDNA generated by reverse transcription of total RNA provides a simple alternative tool for quantitative RT-PCR normalization.
Jiri Libus and Helena Štorchová
Institute of Experimental Botany, Prague, Czech Republic
BioTechniques 41:156-164 (2006)
Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference
genes suitable for normalization

Haller F, Kulle B, Schwager S, Gunawan B, von Heydebreck A, Sultmann H, Fuzesi L.
Anal Biochem. 2004 Dec 1;335(1):1-9.

Selection of reference genes in mouse embryos and in differentiating human and mouse ES cells.
ERIK WILLEMS1, ILEANA MATEIZEL2, CAROLINE KEMP1, GREET CAUFFMAN2, KAREN SERMON2
and LUC LEYNS*,1
1Lab for Cell Genetics, Vrije Universiteit Brussel (VUB) and 2Research Center for Reproduction and Genetics, University Hospital and Medical School of the Vrije Universiteit Brussel (AZ-VUB), Brussels, Belgium
Int. J. Dev. Biol. 50: 627-635 (2006)

Genome-Wide Identification and Testing of Superior Reference Genes for Transcript Normalization in Arabidopsis.
Tomasz Czechowski, Mark Stitt, Thomas Altmann, Michael K. Udvardi, and Wolf-Ru¨ diger Scheible*
Max-Planck Institute of Molecular Plant Physiology, 14476 Potsdam, Germany
Plant Physiology (2005) 139, 5–17

Generic normalization method for real-time PCR Application for the analysis of the mannanase gene expressed in germinating tomato seed.
Dimitris Argyropoulos1, Charoula Psallida1 and Caroline G. Spyropoulos2
1 Institute of Biotechnology, National Agricultural Research Foundation, Athens, Greece
2 Department of Botany, Faculty of Biology, University of Athens, Greece
FEBS Journal 273 (2006) 770–777

Sex-dependent expression of seven housekeeping genes in rat liver.
ASHISH S VERMA AND BERNARD H SHAPIRO
Laboratories of Biochemistry, University of Pennsylvania, School of Veterinary Medicine, Philadelphia, PA, USA
Journal of Gastroenterology and Hepatology (2006) 21, 1004–1008

Housekeeping genes in cancer: normalization of array data.
Khimani AH, Mhashilkar AM, Mikulskis A, O'Malley M, Liao J, Golenko EE, Mayer P, Chada S, Killian JB, Lott ST.
Biotechniques. 2005 38(5): 739-745